Coincident RNA and protein detection...

From: Kim Sipe (kjsipe0@pop.uky.edu)
Date: Tue Mar 09 1999 - 11:38:44 EST


First, thank you for providing a very rich networking resource.

And then I have a question. I've been searching the literature for
information on the use of FISH and immunolabeling for studying co-localized
protein and RNA expression.  I have found some sources indicating that this
is do-able.  I'm wondering, though, what the level of resolution would be
using a confocal scope instead of flow cytometry.  (We'd like to look at a
surface protein, in conjunction with RNA expression, in adherent cells or
tissue preparations.)  I realize many subscribers to this list have
significant imaging and visualization experience beyond flow cytometry, and
I'm hoping someone will be able to fill me in or direct me to an
appropriate source.

Thank you in advance,

Kimberly Sipe
University of Kentucky
Dept. of Anatomy and Neurobiology
Lexington, KY



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