Annexin V

From: silvana (S.Bonfigli@ipsoe.ss.cnr.it)
Date: Wed Mar 03 1999 - 07:22:18 EST

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Hi, we have problem in labeling cells with Annexin V. In our protocol the
cells are resuspended in an incubation buffer which contains 1.5mM CaCl2,
then we add 5 ul of annexin V and we leave on ice for 15'. 
With this protocol we did not observed any positive cell even in the
positive control. Could  anybody give us suggestions?
thank you
Silvana

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