rat epithelial cells

From: ronald hill (ron-hill@sugen.com)
Date: Fri Feb 26 1999 - 16:55:13 EST

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Has anyone used annexin V staining to detect  apoptosis in rat
epithelial cells. We are having trouble obtaining intact cells after
staining.  A majority of the cells become PI positive after removal from
the plate. Any tricks? Should we remove cells first and then stain?

Thanks for any suggestions.

-Ron Hill

Ronald J. Hill, Ph.D.
Group Leader, Immunology Targets
Sugen, Inc.
230 East Grand Avenue
South San Fransisco, CA 94080-4811

Tel: (650) 553-8797
Fax: (650) 553-8313
E-mail:  ron-hill@sugen.com

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