Joe, Here are some of the earlier references dealing with FITC quenching by trypan blue. J Immunological Methods, 60 (1983) 115-124. Differentiation between attached and ingested immune complexes by a fluorescence quenching cytofluorometric assay. J Immunological Methods, 72 (1984) 229-241. Flow cytometric assay for combined measurement of phagocytosis and intracellular killing of C.albicans J Immunological Methods, 101 (1987) 119-125. The use of fluorescence quenching in flow cytofluorometry to measure the attachment and ingestion phases in phagocytosis in peripheral blood without prior cell separation. Good luck, Pam ******************************** Pamela McCloskey Research Scientist II Nassau County Medical Center Department of Pediatrics 2201 Hempstead Turnpike East Meadow, NY, 11554 Phone: (516) 572-6205
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