On Wed, 17 Feb 1999, Richard Meister wrote: > > Hello, everyone: > > I have two questions re: apoptosis that came up during a recent consult on > the subject. > > 1. What is the best positive control to use; i.e., an easy-to-grow cell > line that can be reliably and predictably induced into apoptosis? (I seem > to recall a thread on this question some time ago, but I couldn't find it > in the archives.) I've grown THP-1's and induced apoptosis using camptothecin. With respect to cell lines in general, any cell line that is growth factor dependent will handily undergo apoptosis on growth factor withdrawal. > 2. How should one pronounce "apoptosis"? When I first heard the term > (probably 10 years ago), the second "p" was silent (A-pO-tO-sis). Since > then, I have increasingly heard the second "p" pronounced (A-pop-tO-sis). > And, I can't find the word in a dictionary. Well, I've always pronounced it with an audible second "p". I'm not sure about classical Greek, as mentioned in a previous reply, but certainly in Koine Greek (of 1st century AD) and beyond, all consonants are always pronounced. So, for example, pneumatos would be pronounced with the initial "p" (however difficult that is...). Same with ptosis, and hence apo- + ptosis would be pronounced with the "p" in ptosis audible. Ryan. _/ __/ __/ __/ __/ __/ __/ __/rhung@vcn.bc.ca__/ __/ __/ _Apoptosis=programmed cell death/ __/ \rwhung@interchange.ubc.ca_/ __ _/ --you can't live without it!/ __/ \http://www.vcn.bc.ca/people/rhung __/ __/ __/ __/ __/ __/ __/ \My words Copyright (C) 1998 __
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