On Wed, 17 Feb 1999, Richard Meister wrote: > I have two questions re: apoptosis that came up during a recent consult on > the subject. > > 1. What is the best positive control to use; i.e., an easy-to-grow cell > line that can be reliably and predictably induced into apoptosis? (I seem > to recall a thread on this question some time ago, but I couldn't find it > in the archives.) I generally use a PBL cell line - HL60 (ATCC Desig: CCL 240). These are easy to grow in suspension and can be induces to go into apoptosis by the addition of either: 100ug/ml camptothecin or 100uM Dexamethasone or 50ug/ml etoposide I usually use camptothecin as you should get a reasonable percentage of apoptotic cells after about 4 hours. > 2. How should one pronounce "apoptosis"? When I first heard the term > (probably 10 years ago), the second "p" was silent (A-pO-tO-sis). Since > then, I have increasingly heard the second "p" pronounced (A-pop-tO-sis). > And, I can't find the word in a dictionary. As 'apoptosis' has a Greek origin there is a case for not pronouncing the second p. However English is not Greek and pronounciation can change (1). It seems to me that the second pronounciation is the more common (and the one I use) but I don't know of anywhere where there is a "guide'. http://www.celldeath-apoptosis.org/HistOfApoptosis.html is a good review of the use of the word historically. Derek (1) The "inventor" of margarine originally intended it to be pronounced with a soft g - mar-ja-rine but common usage dictated otherwise. Or is that a UL? **************************************************************************** * Derek Davies Voice: (44) 0171 269 3394 * * FACS Laboratory, FAX: (44) 0171 269 3100 * * Imperial Cancer Research Fund, e_mail: derek.davies@icrf.icnet.uk * * London, UK * * * * Web Page: http://www.icnet.uk/axp/facs/davies/index.html * ****************************************************************************
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