Rick Meister wrote: From: Richard Meister [mailto:meister.1@osu.edu] <mailto:[mailto:meister.1@osu.edu]> Sent: Wednesday, February 17, 1999 2:29 PM To: Cytometry Mailing List Subject: Apoptosis Hello, everyone: I have two questions re: apoptosis that came up during a recent consult on the subject. 1. What is the best positive control to use; i.e., an easy-to-grow cell line that can be reliably and predictably induced into apoptosis? (I seem to recall a thread on this question some time ago, but I couldn't find it in the archives.) 2. How should one pronounce "apoptosis"? When I first heard the term (probably 10 years ago), the second "p" was silent (A-pO-tO-sis). Since then, I have increasingly heard the second "p" pronounced (A-pop-tO-sis). And, I can't find the word in a dictionary. Thanks in advance, Rick Meister To answer your questions: 1. There is a very reliable cell line called CEM-C7 that is a cloned human leukemic T-cell line. It is sensitive to the cytolytic action of glucocorticoids. You may contact E. Brad Thompson here at the University of Texas Medical Branch Galveston at bthompso@utmb.edu <mailto:bthompso@utmb.edu> or you may reference a few of his articles: "Characterization of a Glucocorticoid-sensitive Human Lymphoid Cell Line", Michael R. Norman and E. Brad Thompson, Cancer Research 37, 3785-3791, October 1977 "'Activation-labile' glucocorticoid-receptor complexes of a steriod-resistant variant of CEM-C7 human lymphoid cells", Thomas J. Schmidt, Jeffrey M. Harmon & E. Brad Thompson, Nature, Vol. 286, No. 5772, pp. 507-510, July 31 1980 "Dexamethasone Induces Irreversible G1 Arrest and Death of a Human Lymphoid Cell Line", Jeffrey M. Harmon, et al., Journal of Cellular Physiology, Vol. 98, No. 2, February 1979 We routinely use this cell line in our apoptosis studies and the cells grow rapidly and are very easy to culture. They label very well with Annexin V, CD95, and CD4. 2. If you go to John Kimball's Apoptosis Page at http://www.ultranet.com/~jkimball/BiologyPages/A/Apoptosis.html <http://www.ultranet.com/~jkimball/BiologyPages/A/Apoptosis.html> he writes: " The pattern of events in death by suicide is so orderly that the process is often called programmed cell death or PCD. The cellular machinery of programmed cell death turns out to be as intrinsic to the cell as, say, mitosis. Programmed cell death is also called apoptosis. (There is no consensus yet on how to pronounce it; some say APE oh TOE sis; some say uh POP tuh sis.) Personally I say it with the "p" sound...but, "I say tom-A-to, and you say tom-AH-to..." ----------------------------------------------------------------------- Lisa Reece Research Associate University of Texas Medical Branch Molecular Cytometry Unit Dept. of Internal Medicine-Div. Of Infectious Diseases 301 University Blvd. Galveston, TX USA 77555-0835 Office: (409) 747-1932 FAX: (409) 772-6527 E-mail: lreece@utmb.edu <mailto:lreece@utmb.edu>
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