Does anyone have a working assay to detect microchimerism after BMT by FACS (in the context of immunophenotyping!). I have considered using FISH for Y chromosome, but was unable to develop a method that allowed for hybridization and immunophenotyping simultaneously... Other alternatives include CD31 polymorphism, but I'd rather not use that, and HLA-mismatched staining, which is a pain since you have to have some many different reagents. Has anyone developed a IP-compatible FISH assay or a IP-compatible VNTR assay? Also, is anyone doing VNTR analysis on small numbers of cells (10,000 or less)? thanks for any information mr
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