Saverio, We currently are sorting epithelial carcinoma cells from blood, post surgical using the Ber-EP4 mAb (common epithelial surface antigen). This is a Dako product (Code F 0860). Provided your cell isolation and preparation techniques do not destroy the surface antigen then you might expect accurately detect as little 0.1% epithelial cells, in a cell suspension from the node. We get that kind of detection level, when epithelial cells are mixed in a blood cell suspension. Denis Snider Denis Snider Ph.D., F.C.A.C.B. Associate Professor Dept. of Pathology and Molecular Medicine McMaster University > -----Original Message----- > From: Saverio Alberti [SMTP:alberti@cmns.mnegri.it] > Sent: Tuesday, February 09, 1999 1:24 PM > To: Cytometry Mailing List > Subject: Re: Epithelial cells in lymph node > > > PCR for any epithelial-specific mRNA will do. People are doing that for > breast carcinoma (Her-2/neu). > > Saverio Alberti > Head, Unit of Experimental Oncology > Department of Cell Biology and Oncology > Consorzio Mario Negri Sud > 66030 Santa Maria Imbaro (Chieti), Italy > Phone: (39) 872-570.293 > FAX: (39) 872-578.240 > E-mail: alberti@cmns.mnegri.it > > > On Tue, 9 Feb 1999, Graeme Chapman wrote: > > > Hi everyone > > > > A surgical colleague wants to know if there is a method to detect minor > numbers of epithelial cells in a lymph node by flow. He is particularly > interested in nodes where there is no apparent sign of epithelial cells > observable by microscopy. Any suggestions or methods would be appreciated > > > > Graeme Chapman > > gvchapman@bigpond.com > >
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