>Does anyone know of any good cell cycle analysis software? >We are currently moving from the HP workstation to the >Macintosh workstation, and find that Modfit is a very >poor software application. We really liked CellFit for the HP, but >BD doesn't do Cell Cycle analysis software anymore, since they >can't make any money at it. We've also heard of Phoenix flow >systems MacCycle. We were just wondering if there were any >others on the market besides those two applications. Any >help is greatly appreciated. Thank You. > FlowJo, originally developed here at Stanford, now comes equipped with an integrated cell cycle platform. It fits both Watson's "Pragmatic" model as well as the Dean-Jett model (with the Fox modification). Using an interactive interface, you can put constraints on many of the fitting parameters, allowing you to model most DNA distributions. However, there is currently no way to model debris background subtraction (a process which is controversial), nor can it model cell divisions (using dyes like PKH-26 or the like). In other words, it's not as sophisticated as ModFit, also available for the Mac, but suffices for probably 90% of the users out there! FlowJo integrates this platform into the unique drag-and-drop interface that lets you apply your customized models to entire experiments in a matter of seconds, create graphical reports in which you include statistics, keyword information, and so forth. You can even create template analyses, so that subsequent experiments can be analyzed just by loading the data into the workspace. For more information, see the FlowJo web site: www.treestar.com/flowjo mr
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