monocyte seperation

From: Mats Alheim (Mats.Alheim@cnsf.ki.se)
Date: Thu Feb 11 1999 - 07:11:06 EST


Hello,

I am working with immunology of MS, mainly with cytokines.
I will study cytokine expression by monocytes. I have problems with
monocyte seperation from PBMC. I will have max 20 ml of blood from each
patient and I need around 1 million monocytes.

If you can help me with monocyte seperation techniques, I would appriciate.

With best regards

--------------------------------------------------------
Mats Alheim	
Huddinge University Hospital
Division of Neurology	
S-141 86 Huddinge, Sweden	

Phone Int. + 46 8 585 822 28
Fax Int.+ 46 8 585 870 80
E-mail: Mats.Alheim@cnsf.ki.se



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