Dear Flowcyte gurus, First I would like to thank all for their good advice with paraformaldehyde. I found this List a week ago and really appreciate the quality of the discussion and information. I already searched the archive for my other question, but found nothing about it. So here is my question : I am studying the expression of constitutive GFP in mammalian cells. GFP will leak only from dead cells when membrane integrity is loss (personal observation). As paraformaldehyde permeabilize the cell membrane, will the GFP expressed will be entrapped into cells or will GFP leak with time... Is there any way to prevent the leakage of GFP after fixation ? Does high paraformaldehyde concentration could be the solution (4%, 6%, 8% ?). Thank you for your help. Philippe-Alexandre Philippe-Alexandre Gilbert Institut de recherche en biotechnologie Conseil National de Recherche du Canada 6100 Royalmount, Montreal, Quebec (Canada) H4P 2R2
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