Greetings Fellow Flow-ers, > > I have a customer whose query is pasted in verbatim below - it is > completely outside my experience and I would be very grateful for an > answer from anyone who may be able to help. > > "Syncytiotrophoblasts (placental cells) do not proliferate therefore I > would imagine, I would get a different picture than expected. The way they > differentiate is by fusing with each other. Therefore, during the > analysis, am I likely to have the same signal of a tetraploid and the two > diploids cells together at the same point on the graph? Will the peaks > of syncytia going to be flatter than expected in comparison with the > tumour cells when tested?" > > Technically, the DDM is 'ON', the customer is using PI on Fl2-A and/or > Fl2-W, at low flow rates, with suitably dilute samples. > > My guess is that fused syncytiotrophoblasts will appear in the same > position as tetraploid/polyploid cells, but with little showing between > them and 'resting' G0 cells - is this a sensible assumption? In the > meantime, I've suggested that she sort some peaks and have a look at them > under a microscope. > > With regards and best wishes > > Nigel Llewellyn-Smith > BD UK Ltd > Between Towns Road > Oxford > OX4 3LY > > Tel +44 1865 748 844 > Fax +44 181 992 8662 >
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