Dear Cytometry group, I am working with mammalian cells and studying the expression of GFP on FACS. During the preparation of my samples, I had a problem of aggregation. Is there a way to separate individual cells from aggregates "after fixation of paraformaldehyde" or is it to late to do so. Thank you for your help. Philippe-Alexandre ---------- > From: DIANE M SHARP 695-7248 <Diane.M.Sharp@dupontpharma.com> > To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> > Subject: Question from a co-worker > Date: Monday, February 01, 1999 8:32 AM > > > Dear flow group, > > Someone I work with has posted a question for me, so I'll pass it on to you. I > will welcome your replies. Write me at email address: > Diane.M.Sharp@dupontpharma.com > The question is posted below: > > Dear People, > > I am very interested...for career development...in attending some workshops and > meetings in opitcal cell analyses techniques. This includes all FACS and > confocal microscopy methods. > > Would you happen to know of meetings or training programs in these areas? I am > interested in any techniques were I could use the single chain antibodies I > make a probe to look at cell surface or internal (through permeabilization) > protein dynamics. > > Thanks for your time, > > Gary
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