We use FITC labelled F4/80 from Serotec to label murine macrophages. It won't label monocytes or dendritic cells, only macs. If you want to pick up other phagocytic cell populations, I would suggest CD11b. kb -- ----------------------------------------------- "Kids, you tried your best, and you failed miserably. The lesson is, never try." --Homer Simpson Keith Bahjat Graduate Assistant University of Florida College of Medicine Gainesville, Florida kbahjat@ufl.edu ---------- >From: "Martin Lehner" <lehner@tycho.gk-biochem.uni-konstanz.de> >To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> >Subject: phagocytosiswith murin cells >Date: Fri, Jan 29, 1999, 7:50 AM > > > Hello, > I am trying to establish an assay for the determination of > phagocytosis and oxidative burst by mouse immune cells (whole blood, > peritoneal lavage cells, bone marrow cells and spleen cells). > Unfortunately I have problems in finding appropriate antibodies to > distinguish different populations (especially no marker for > macrophages!) > I hope someone has already worked with murine cells and can give me > some information or protocols. > Thanks, > Martin Lehner > University of Konstanz > Germany
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