Re: phagocytosiswith murin cells

From: Keith Bahjat (kbahjat@ufl.edu)
Date: Tue Feb 02 1999 - 00:18:33 EST


We use FITC labelled F4/80 from Serotec to label murine macrophages. It 
won't label monocytes or dendritic cells, only macs. If you want to pick up
other phagocytic cell populations, I would suggest CD11b.

kb
--
-----------------------------------------------

"Kids, you tried your best, and you failed miserably. The lesson is, never
try." --Homer Simpson



Keith Bahjat
Graduate Assistant
University of Florida
College of Medicine
Gainesville, Florida
kbahjat@ufl.edu


----------
>From: "Martin Lehner" <lehner@tycho.gk-biochem.uni-konstanz.de>
>To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
>Subject: phagocytosiswith murin cells
>Date: Fri, Jan 29, 1999, 7:50 AM
>

>
> Hello,
> I am trying to establish an assay for the determination of
> phagocytosis and oxidative burst by mouse immune cells (whole blood,
> peritoneal lavage cells, bone marrow cells and spleen cells).
> Unfortunately I have problems in finding appropriate antibodies to
> distinguish different populations (especially no marker for
> macrophages!)
> I hope someone has already worked with murine cells and can give me
> some information or protocols.
> Thanks,
> Martin Lehner
> University of Konstanz
> Germany



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