Hello: Hopefully, someone out there can answer my question: When looking at propidium iodide uptake for cell viability, do you typically see a large shift in fluorescence in the FL-2 channel? It has been my experience that non-viable cells are very bright (3 log shifts over auto), with a characteristic change in scatter properties. Someone recently showed me data where they claim that a slight shift in fluorescence (< 1 log) of all the cells indicates 100% cell death. I find this hard to believe, since the experiment consisted only of cells + antibody incubated @ 4oC. If this is not cell death, then what can account for the slight uptake of PI? Anything you can tell me would be greatly appreciated! Thanks, Laura Vitale Medarex, Inc. Annandale, NJ
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