Mouse bone marrow/Mac-1 and Gr-1

From: Eduardo Rego (edumrego@hotmail.com)
Date: Wed Jan 27 1999 - 11:58:58 EST


Hi,
I need to sort promyelocytes from mouse bone marrow, I was thinking in 
sorting cells with high FSC, CD33+ and HLA-Dr -. Does anyone has any 
suggestion ?
Moreover, should I consider cells Mac-1 + and Gr-1 + less mature than 
those presenting just one of these markers ? Would anyone suggest a 
panel of Mabs for the analysis of the myeloid compartiment in the mouse 
bone marrow ?
Thanks in advance.
Eduardo Rego
Memorial Sloan Kettering 
New York

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