I apologize, I did not make myself clear. The information we want on PI is for staining slides. Not Flow Cytometry. The user came to me because she has used PI on the Flow for DNA analysis, and thought I would be able to help with general info on the dye,ie quenching time. She really wants to know the quenching time/potential of PI because she needs to put it under a microscope and is curious whether or not the light from the scope will quench the fluorescence before she can look at the slide. I realize this isn't a true "cytometry" question, but I thought somebody might just know the answer. She was also curious as to the quenching time of PE because she thought it may be better to work with than PI but less light sensitive than FITC which she has tried to stain slides with, but is having problems with it fading. Sorry for the confusion, and once again, Thanks in advance, Joan K Flow Cytometry Lab Supervisor VMRCVM (540) 231-4115 FAX 540-231-7367 jkalnits@vt.edu "It is better to serve than to receive." B. Borg
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