Dear Flowers, I have been asked to sort (mouse) skin fibroblasts ... and have never done so. Would you be so kind as to offer me a protocol for the isolation of fibroblasts from skin for the purpose of sorting, and any relevent advice. Also, what would be the best way to identify these fibroblasts? My question: is there a method to isolate/purify such cells without sorting? Thanks to all. Claude Claude Cantin Director, Flow Cytometry Arthritis and Immune Disorder Research Center Toronto Hospital Toronto, Ont.
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