Dear Flow cytometry group, I did not get a HUGE response to my request for comments of a cytometry article (Cytometry 33,385,1998). I got only three. However, I felt that I should try to summarize the comments without giving the names. 1. Studies with CAP results were not able to relate %S value differences between labs based on defined procedures or instruments. Therefore, each lab must really do their own clinical trial or at least define their own distribution for the comparative studies. 2. These studies only used frozen tissues. Would the results be any different if the samples were from paraffin embedded tissues. 3. The mathematical manipulations seem to be unnecessary for clinical application and might in fact introduce additional variability into the studies since the setting are highly subjective. This is clearly a novel idea that needs to be proven by other laboratories. 4. The correct fitting of DNA histograms is difficult and one must always use the same model with the same type of tissue to get the best results. 5. One should use proliferative fraction (S + G2M) rather then %S alone in these studies. 6. These studies do not take into account that aneuploid cells have significantly higher %S then diploid cells. It would seem that this is really a ploidy study rather than a %S study. It would have been nice to break the groups into diploid and aneuploid and then repeat their analysis. This was not discussed in the paper. 7. Can these results be extrapolated into other tumors? 8. An exclusion rate of only 9% seems very good. Use of paraffin sections would give a higher exclusion. What were the reasons for exclusion in their studies? If there are more comments, please submit them to the board and thanks to those who did take the time to give their comments. Dr. Timothy Kute Telephone # = 336-716-2950 Associate Professor in Pathology email = tkute@bgsm.edu Wake Forest University School of Medicine Medical Center Blvd Winston-Salem, NC 27157-1072
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