I am new to flow and have some basic questions and some specific questions. I would like to examine antibodies to the Epo receptor by flow. I purchased a goat antibody that recognizes soluble receptor in ELISA and western. When I try to examine it on cell surface on TF-1 cells, a human erythroleukemia cell line, I do not detect a signal with either FITC or PE conj secondary antibodies. Can anyone tell me the lower limit for detection of a cell surface protein by FACS? How many receptors per cell do I need to see binding? Also, has anyone out there ever examined EPO receptor by FACS? Can you help me? Thanks, Kathy Bowdish Prolifaron, LLC
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