Hi all, A researcher at my institution says he saw a kit for detaching cells in a science mag but has not been able to relocate it. If anyone knows of such a kit I would like to hear from you. We are trying to detach an intestinal cell line from flasks. We used EDTA along with sharply tapping the flasks on a countertop...the cells detach...but the viability using PI exclusion was very low. Any suggestions of a less harsh method are welcome..we can't use trypsin because we want to assay for a surface receptor. Thanks, Mike Tanner U of Md Vaccine Dev 410-706-7376 mtanner@umaryland.edu
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