We routinely use CD83-PE from Immunotech for staining our monocyte derived dendritic cells. It looks as good as CD83 can possibly look! -- Keith Bahjat University of Florida College of Medicine Department of Pathology Gainesville, FL 32607 kbahjat@ufl.edu Yu-Min Huang wrote: > Dear collegues, > > I am working with dendritic cells (DC) and I have been using pure CD83 and > secondary conjugated antibody for flow cytometric assay. I wonder if > somebody use direct conjugated CD83 (anti-human) for DC. It would be > grateful you reply to me. > > Yu-Min Huang > Div. Neuroimmunol > R54 Huddinge Hospital > 141 86 Stockholm, Sweden > Tel: 46 8 5858 2258 > Fax: 46 8 5858 7080
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