Dear Cytometrists! I am doing some 7AAD staining of lymphocytes for flow. I am not sure whether I should wash 7AAD out before analysis or keep the cells in 7AAD. Strangely, keeping 7AAD increases background, but washing reduces DNA staining intensity. Is 7AAD's binding to DNA reversible and how stable it is? What final concentration of 7AAD are you using? Waiting impatiently for you response, Robert Gniadecki Dept. Dermatology, University of Copenhagen, Denmark ______________________________________________________ Get Your Private, Free Email at http://www.hotmail.com
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