Hello Fellow Sorters, I know this topic has previously been discussed, but we have not had post-sort cell death until recently and previous suggestions do not really apply here. Three months ago we moved our Vantage into our new facility. The only difference between the old set-up and the current one is the fact that we are using a chiller to cool the laser. Nothing else. Same 3% H2O2 sterilization procedure, same sheath fluid (PBS, Ca++, Mg++, pH 7.2) but different water source, and no temperature problems. Sorts are going very well but cells put into culture experience apoptosis within two days. We've done the control experiments (unsorted, unstained cells; unsorted, stained cells; and sorted, stained cells). The only ones that died were the sorted cells. So... any ideas, suggestions, and prayers would be greatly appreciated. Thanks, Elizabeth T. Kraus et.kraus@baylordallas.edu
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:52:56 EST