IFN-gamma was the first (I believe) cytokine to be examined by flow by Dave Ferrick and co-workers: (Nature 1995 Jan 19;373(6511):255-7 Differential production of interferon-gamma and interleukin-4 in response to Th1- and Th2-stimulating pathogens by gamma delta T cells in vivo. I think that people will find that they get much better responses from the list members if they ask better (ie more specific and more informed) questions. While there is no substitute for personal experience, its probably helpful to check out the literature in a field first, then ask about what protocol's and positive controls perople use, etc. More and more, I think, people are using this list instead of the library. I have been wondering if we could compile some FAQ files (and weblinks) as a mechanism to point people in the right direction and to share the weallth of knowledge represented by this community. Anyone interested ? Rachel M. Gerstein, Ph.D. Dept. of Mol. Gen. & Micro. University Of Massachusetts Medical School 55 Lake Avenue North Worcester, MA 01655 508-856-5920 (FAX)
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