Dear Collegue Illingworth, I haven´t seen a CD23+CD8+ T-NHL so far, neither in our cases nor in NHL classification schemes, talks or literature. The subset analysis is not typical for a leukemic lymphoma. CD4/8 is like kappa lambda for polyclonality. Obviously the clinical information is insufficient and completely against CLL. The request for CLL is strange in the light of the current blood picture. May be some days ago there was a significant reactive CD8 lymphocytosis driven by a viral infection that now suppresses myelopoiesis in the bone marrow. It would have been desirable to prove this by CD57 and CD38 on CD8 or by incresed HLA-DR on monocytes (and morphology). As only 15% of lymphs are involved it will be hard to identify these in the blood smear but I recommend you should always try to. First of all you may want to contact the physician who is in charge of that patient to obtain further clinical information (fever, lymph nodes, time course etc.). I found these contacts were mostly positive and resulted in a positive feed back to the flow lab. Strange coexpressions occur and might be either artificial (eg. like anti-mouse Igs as discussed in the past here on the server or in cytometry) or related to autoimmmune, atopic (FceRI on monocytes etc.) or infectious diseases (eg. CD4 epitope on CD8 cells in HIV). Regards Thomas Nebe Universitätsklinikum Mannheim D-68135 Mannheim, GERMANY thomas.nebe@ikc.ma.uni-heidelberg.de > > > ______________________________ Reply Separator > _________________________________ > Subject: CD23 expression on T-cells > Author: dcdsflow@mint.net at INTERNET > Date: 29/12/1998 18:03 > > We just analyzed the peripheral blood of a 52 year old gentleman which > came > to our lab for a CLL work-up. > WBC: 2.0 RBC: 4.21 HGB: 13.9 PLT: 54 19% grans 66%lymphs 7% > monos/eos > 8% ATL's > > As you can see, the patient has a definite neutropenia/thrombocytopenia > and > not an absolute lymphocytosis but we ran it anyway. The flow analysis of > the brightly CD45+ lymphocytes showed approx. 94% T-lymphocytes (CD2+, > CD3+, > CD5+, CD7+) with 48% CD4 and 44% CD8. 5% B-cells were also identified > with > 2% kappa and 2% lambda. However, about 15% of the lymphocytes also > exhibit > CD23/CD7, CD23/CD3 and CD23/CD5. There is no CD19/CD5 coexpression! The > CD23 expression was not seen on the CD4 lymphs. I couldn't perform the > CD23/CD8 and the CD23/CD2 because we don't carry this color combination > but > I am assuming that these cells are also pos for CD2 and CD8. I am really > at > a loss since I have never seen this strange coexpression. Is this just > atypical or abnormal (?malignant)? > > We appreciate your input > Andrea Illingworth > Dahl-Chase Diagnostic Services/Flow Cytometry > 333 State Street > Bangor, Maine 04401 > (207)990-4855 > > > > ______________________________ Reply Separator > _________________________________ > Subject: 1998 was a good year > Author: jpr@flowcyt.cyto.purdue.edu at INTERNET > Date: 31/12/1998 02:43 > > > Colleagues: > 1998 was good year I think. In the next few months it will be approaching > 10 years since the Cytometry list began. We are still going and still > doing > cytometry!! On average, we added 4 people per week to the list. The > quality > of interaction remains high and members show a great deal of respect for > each other. I think Cytometry (discussion) is almost unique in its high > standards! > > Last week, I ventured into the internet to check out some list-serves... > well > there are about 18,500 discussion groups listed .... I went into several > and > found a lot of trashy discussion, petty peeves, spiteful remarks and > little > good material... I know, I only sampled a few (all in distance learning) - > but > what I saw, I did not like. Long live Cytometry. > > All of us here at Purdue University Cytometry Laboratories, the internet > home of the CYTOMETRY discussion, wish you a prosperous 1999. > > Paul Robinson > > J.Paul Robinson, PhD PH:(765)4940757 > Professor of Immunopharmacology > Purdue University FAX:(765)4940517 > EMAIL:jpr@flowcyt.cyto.purdue.edu > WEB: http://www.cyto.purdue.edu > > > > ______________________________ Reply Separator > _________________________________ > Subject: Re: Chemdex reconsiders...but > Author: jpr@flowcyt.cyto.purdue.edu at INTERNET > Date: 04/01/1999 20:17 > > > Mark Corio's original note about chemdex sent me scurrying to their site > to > check it out. > My reaction was....... painful. Anyone who makes it so hard to get at > information which is already free and available from each vendor is > on thin ice.... > > What Chemdex is, is a way to find products from wnywhere and > purchase them from one place - just like INSIGHT is for computer > products. It remains to be seen if scientists will accept "biological > parts" in a similar way that the system works for computers... > > They have put a rather experienced team together if you check out > their Board of Directors.... so .....somebody thinks there's plenty of > money in research labs.... > > Paul > > J.Paul Robinson, Ph.D., Professor of Immunopharmacology > Director, Purdue University Cytometry Laboratories > Purdue University, West Lafayette, IN 47907-1515 > (Ph) (765) 494-0757 Fax (765) 494-0517 > jpr@flowcyt.cyto.purdue.edu > http://www.cyto.purdue.edu
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