Kirk Watkins wrote: Is anyone using a calculated ratio for displaying Indo-1? The instrument does not have the ratio parameter but does have the optics, laser, etc. for running Indo-1. Can FCS Assistant be used to modify the data file to add the ratio parameter and is this valid for measurement? Any and all help would be appreciated. Kirk The beta version of Flowjo, and Multitime, by pheonix software will calculate software ratios. Excellent correlation with ratio calculated by pulse processing (Flowjo, don't know about Multitime, but do not know why it would not be the case). ron Ronald L. Rabin, M.D. Laboratory of Clinical Investigation National Institute of Allergy and Infectious Diseases National Institutes of Health Building 10/Rm 11N228 MSC 1888 10 Center Drive Bethesda, MD 20892-1888 Phone (301) 402-4910 FAX (301) 496-7383 email: rr84g@nih.gov
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