Padma writes: "Has anybody out there tried using fluorogenic substrates to measure caspase 3 activity during apoptosis by flow. Any help with info on reagents and vendors will be greatly appreciated." ____________________________________ We had a go using the Biomol AMCA-DEVD fluorigenic substrate for caspase 3 (aka CPP-32/yama). It gave a great fluorescence signal, but it all washed out of the cells. In their catalogue Biomol mention that they are working on a probe that yields a product with better cell retention. I think this would be a very useful reagent if it could be made to work. It's time we had some reagents for flow enzymology that measure things of real biological interest! David Hedley Ontario Cancer Institute Toronto
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