Dear Dr. Koji Saeki, There are a number of anti-human CD34 antibodies that have been claimed to cross-react with non-human primate stem/progenitor cells. However, in most cases, these-cross reactions are so weak as to be virtually useless for most purposes. Fortunately, there are still one or two that may be useful. Antibody 12.8 (IgM) was the first anti-CD34 antibody (detects a class I epitope) that was shown to be capable of selecting non-human primate stem/progenitor cells (Berenson and Andrews et al, J. Clin. Invest.81:951, 1988) and can be conjugated with Biotin and used with fluorochrome conjugated streptavidin to detect non-human primate (including Cynomolgous) CD34+ cells. More recently, another clone called 563 (a class III reactive epitope) has been developed that detects a variety of non-human primate (including Cynomolgous, RG Andrews, personal communication),CD34+ cells. This clone was developed by Gaudernack and Egeland (Leukocyte Typing V. Schlossman et al Eds, Oxford University Press, 861-864, 1995). This reagent can probably be directly conjugated with a variety of fluorochromes and used directly in your studies. Further information on the above can probably be obtained directly from Bob Andrews (<randrews@fred.fhcrc.org>) and Torsten Egeland (torstein.egeland@labmed.uio.no). I hope this is helpful, Rob Sutherland Oncology Research, The Toronto Hospital
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