Cell cyclers: two questions-- 1. When viable cells are labeled for RNA content with pyronin Y and sorted and returned to culture, how long does the stain remain associated with the cells? I ask because of some concern that pyronin fluorescence might interfere with subsequent antibody staining and analysis. 2. Is it possible to read pyronin Y staining after fixing with 0.5-1.0% paraformaldehyde? Thanks for the help. Doug Dooley, American Red Cross, Portland, Oregon deekewb@AOL.com
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