Our average transplants (allo + auto) per year are bone marrow n = 170 peripheral blood n = 390 cord blood n=12 We are using both ISHAGE and a three color panel for subset analysis. We have found excellent correlation between the two methods for total CD34 quantitation and useage of methods becomes protocol driven. The ISHAGE is used for stat CD34 levels to evaluate donor/patient mobilization to determine when to begin collection, quantitation of CD34 in collection products prior to various purging techniques, or reference vial thaws to QC transfusion products in which case we also include propidium iodide to monitor 'damaged, non-viable' cells. The majority of our CD34 evaluation is a series of protocol specific three color panels for subset evaluation. April Translational Research & Flow Cytometry Laboratory Blood & Marrow Transplantation Univ Texas MD Anderson Cancer Center
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