Hello, I have a problem concerning the interpretation of annexin V positive cells analysed by CMF. The cells are not trypsinized, but released from the tissue by collagenase digestion, which probably does not damage the cell membrane. How can we explain the increase, with the culture time, of the percentage of annexin V positive cells (reaching 78%), whereas TUNEL method gives always negative results (TUNEL used as a routine method in our laboratory on other cell types) ? Thank you for any help ! @@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@ Odile Sabido Faculte de medecine Jacques Lisfranc Centre Commun de Cytometrie en Flux 15, rue Ambroise Pare 42023 Saint-Etienne cedex 02. Tel : 33 (0) 4 77 42 14 41 Fax : 33 (0) 4 77 42 14 89 sabido@univ-st-etienne.fr web site http://www.univ-st-etienne.fr/cccf/ @@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:50:26 EST