Hello, I am posting this as a favor to a friend not currently on line.....please address any responses to me and I'll forward them on thanks..... Dave G. We are currently involved in efforts to measure lipid content in single-cell marine organisms. To achieve this, we would like to use Nile Red, a stain that is soluble in acetone. Our question is this- Is there a safe concentration of acetone that can be used to acheive a reasonable signal in our biological sample that may be run on a FACScan? Are there alternative solvents that would be more forgiving to the FACScan plumbing? Any helpful comments would be greatly appreciated!! > Dave Gebhard--Director FACS Facility Albert Einstein College of Medicine Chanin Building 309 1300 Morris Park Avenue Bronx, New York 10461 718-430-2724/ 3573
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