We have a homemade device in our inverted microscope. It's a chamber made with plastic (metacrylate) with holes for the control screws and as air heater. It has not humidity control and not CO2 control, but if you work with bottles it is not necessary. Just a detail. In the way of the light we put a piece of glass linked to the plastic to avoid problems with the transparency of the plastic. Hope it helps, >From: "Raymond B. Hester" <rhester@jaguar1.usouthal.edu> >Dear group, > >We want to place cells on an inverted-microscope stage and observe >changes in fluorescence using a PTI system (stage will not be moving during the >experiment). Can someone tell us of a (cheap) source for a reasonably >accurate sample warmer (37 C)? Our price range is approx. $500. Any ideas? > >TIA > >Ray > >++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ >Raymond B. Hester, Ph.D. >Flow Cytometry Lab >CSAB 357, Biotechnical Services >The University of South Alabama >Mobile, AL 36688-0002 >Email: rhester@jaguar1.usouthal.edu >Voice: (334) 460-6029 >FAX: (334) 460-6073 >++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ > > Jose C. Segovia Dpt. of Molecular and Cellular Biology CIEMAT Av. Complutense, 22 28040 Madrid SPAIN Tfn : 34 1 346 6484 Fax : 34 1 346 6393 E-mail : Segovia@ciemat.es
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