I would like to know if there is evidence that cells going into apoptosis can also continue to synthesize DNA. In our animal system we observe that the majority of the primary B cells during 3 day culture are in G2M by PI staining by 24-48 hrs and in apoptosis by TUNEL by 48-72 hr. [H]-thymidine uptake indicates that DNA synthesis is occuring and peaks at 72 hr. ([H]-thymidine in culture last 18 hrs). Duel staining for cell cycle and apoptosis shows that it is the G2M cells that are in apoptosis..... Peak thymidine uptake at 72 hrs when most of the cells are in S/G2M and apoptotic in G2M suggests that these appoptotic cells are continuing to synthesize DNA.
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