Hello Ken Re - degrees of freedom in calculating p from K-S stats . . . . . >>Doing this will result in any two histograms that are not identical being highly statistically significantly different. In other words using channels as degrees of freedom makes the K-S test ridiculously too sensitive to trivial differences in the histograms.<< Empirically - I noticed this (overly sensitive) in BD's implementation in CellQuest. As I said in a previous post - I'm not too fond of just accepting someone else's interpretation of a technique in implementing a software package - hence wanting to get to the method for my own critical interpretation. >> I am sure there are others listening to this discussion who are better qualified to discuss how one calculates degrees of freedom for a flow histogram - I for one would be interested in such a discussion.<< Me too! Please post/copy to me any discussion arriving directly to you which is not copied to the Cytometry List (I will reciprocate). Robin Barclay SNBTS, Edinburgh e-mail:Robin_Barclay@compuserve.com Web: http://ourworld.compuserve.com/homepages/Robin_Barclay
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