Hi all Flowers Our laboratory has been trying to perform a qualitative agglutination assay for Bordetella Pertussis in pre and post vaccinated sera using a visual end point. The results are not satisfactorily reproducible from one reader to another. The end point is very subtle; a fine microagglutination that is recommended to be read under oblique light with a magnifying glass. Have any of you any experience with bacterial agglutination by flow. We have a Coulter XL; I have no prior experience with 'bugs' and would need some guidance as to recommended settings etc. I am VERY keen to eliminate the human error apparently inherent in this assay...am I asking the impossible!!!?? Jane Hughes Chief, Institute of Child Health Lab, Red Cross Children's Hospital Rondebosch, Cape Town 7700. South Africa Tel 021 658 5315 Fax 021 689 1287
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:50:21 EST