Hello everybody, We use a kappa/lambda/CD19 triple to establish clonality for B cells, but we are noticing that some of our cases of Hairy cell leukaemia appear "double stained" for kappa and lambda. We use 2% bovine serum albumin in our wash buffer and have tried blocking with various reagents, but no luck so far. Has anyone come across this problem, or have any ideas? Thanks in advance Steve Couzens (Couzens@cf.ac.uk)
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:50:20 EST