Keith, Try UV excitation of cis/trans(?) parinaric acid? Larry Sklar did some work on this lipid, but I don't recall if it was by flow. Bob -----Original Message----- From: Keith Bahjat [SMTP:kbahjat@ufl.edu] Sent: Thursday, November 13, 1997 1:51 PM To: Cytometry Mailing List Subject: Lipid staining I am attempting to stain neutral lipid bodies within dendritic cells for the purpose of enumeration (per cell, as well as cells containing inclusions) and am having trouble finding an adequate procedure. I have tried Nile Red with and without glutaraldehyde fixation of the cells, but the background is too high to delineate positive from negative. My hope is to find a narrow bandwdth dye that will allow simulataneous detection of typical fluorochromes, but I'll take what I can get. Any suggestions appreciated. Thanks. -- Keith Bahjat Graduate Assistant University of Florida College of Medicine Gainesville, Florida kbahjat@ufl.edu
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:50:20 EST