Hi Mayuni, Try Dexamethason 1 uM to make your thymocytes apoptotic. After 3 hour of culture about 30% of your thymocytes are Annexin V positive and PI negative. About 5% is positive for both dyes. When you do the incubation longer than 3 hr, you get more apoptotic cells. My suggestion is, make a time study an measure after every hour the annexinV / PI . After this you can make your choice what time you use in the future. Succes Cor Jacobs Department of Nephrology University Hospital, Nijmegen Nijmegen, the netherlands E-Mail: C.jacobs@nefro.azn.nl
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:50:19 EST