FITC & PE

From: Vivian Wu (ffwu@unixg.ubc.ca)
Date: Fri Nov 07 1997 - 14:22:25 EST


Hi, Fellow Flow-ers,

Thanks so much for all your help on my trivia question. I am now
enlightened! From your answers, there are 3 forms of phycoerythrin,
B-phycoerythrin from blue green algae, C-phycoerythrin from cyanobacteria
and R-PE from rhodophyta (and one who thinks "R" stands for Rhodamine)
with R-PE better matched for the argon laser found on "normal flow
cytometers". The expert in this field is Alexander Glazer from Berkely if
anyone needs to look up references. 

As for FITC it is the structural formula that matters and my search into
www.probes.com again turned up FITC (Isomer I) as C21 H11 NO5 S MW 389.38

Thanks again.

Vivian Wu
Immunology Lab.
Vancouver Gen. Hospt.
B.C. (email: ffwu@unixg.ubc.ca)



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