Sorting HIV+ on FACSCalibur

From: Abby Allen (allena@helix.mgh.harvard.edu)
Date: Thu Nov 06 1997 - 16:07:00 EST


Hello!

We would like to sort unfixed HIV+ samples using our FACSCalibur and the
cell concentrator.  I am under the impression that the FACSCalibur is a
completely closed system and should not create aerosols.  I have called BD
and was told they thought it should be ok.  They are checking into it.  I
"think" it will be ok too, but I need more reassurance than thinking - I
need to know it will be safe.  Anyway - is there anyone who has done this
and has documentation as to the safety (or not) of this instrument?  

Thanks in advance for your help!
Abby Allen
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Abby Allen
Flow Cytometry Laboratory
Warren 515
Mass. General Hospital
75 Charles Street
Boston, Ma  02114

(617)726-8487
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