On Tue, 21 Oct 1997, MK wrote: > Try this: > While measuring Fl1 against Fl2 add 15 microliters of a 25 micromolar > solution of calcium ionophore. Look at your cells, within 1-4 minutes > you'll see the 405 nm emission go up. If you don't see this it means > you don't have any Indo-1 in your cells and you're measuring autofluorescence. > Good luck! > M. Kushnir > mkushnir@playfair.utoronto.ca > Thanks to your informative replied answer. I'm sorry that I was a bit late to reply it. I've done the experiment as you suggest, and it shows that no emission going up. In my case, I use Fluo-3 instead of Indo-1. Do you have any idea how to make more Fluo-3 inside the cell? Thanks in advance. Best Regards, Marianti M.
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