Does someone know any non-permeabilizing cell fixation method (to avoid leaking plasma membranes) to be used on cells that will be analyzed by FACS. I will appreciate any suggestion and/or protocol on the matter. Thank you in advance, Ernesto. -------------------------------------------------------- Ernesto Oviedo Orta, M.D -------------------------------------------------------- Medical Research Council Intercellular Signaling Team, Department of Medical Biochemistry, University of Wales Coll. of Medicine, Heath Park, Cardiff CF4 4XN, U.K e-mail: oviedo-orta@cardiff.ac.uk Direct Line: 01222-742284, 01222-742802 Fax: 01222-744905, 766276.
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