Wanda-- We did some work in this area some years ago that you might find helpful: Cupp, J.E., Leary, J.F., Cernichiari, E., Wood, J.C.S., Doherty, R.A.:"Rare Event Analysis Methods for Detection of Fetal Red Blood Cells in Maternal Blood" Cytometry 5: 138-144 (1984). Corsetti, J.P., Cox, C., Leary, J.F., Cox, M.T., Blumberg, N., Doherty, R.A.:"A Comparison of Quantitative Acid-Elution Technique and Flow Cytometry for Detecting Fetomaternal Hemorrhage" Annals of Clin and Lab Sci 17(3): 197-206 (1987). Good luck. James F. Leary, Ph.D. Professor of Internal Medicine Chief, Molecular Cytometry Unit Division of Infectious Diseases Tel: 409-747-1927; fax: 409-772-6527 On 10/24/97 Wanda wrote: We are looking at performing quantitation of D positive cells (FMH) by flow cytometry. I have been trialing Fluro-D (FITC labelled, CSL product) using the method as recommended by the manufacturer. So far I have found that my standard curve results (O Neg blood spiked with O Pos blood) don't always correlate as close as I would expect/like. Is this due to poor technique or have other operators found similar results. So far, I have been able to detect low levels of D positive cells with minimal background. However, I would appreciate any comments from other users about potential pitfalls, correlation to Kleihauer results, best method of reporting (% or mls of D cells) and any other general hints.
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