Aside from the problems mentioned about the ways that a cytometer can slurp up cells, there is always the possibility (probability?) that the user has counted cells with a hemocytometer *BEFORE* staining and washing and resuspending. It's amazing how many cells get lossed during the staining procedure, especially when you start out with low numbers. Before you blame the cytometer, try counting the cells again after staining. (This also helps in assigning blame for low cell recovery after sorting!) Alice Alice L. Givan Englert Cell Analysis Laboratory Dartmouth Medical School Lebanon, New Hampshire NH 03756 USA tel 603-650-7661 fax 603-650-6130 e-mail givan@dartmouth.edu
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