NIH 3T3 surface antigen

From: RICHARD L. DARLEY (Darley@cardiff.ac.uk)
Date: Tue Oct 21 1997 - 06:44:35 EST


I'm looking for an antibody which will distinguish NIH 3T3 cells
from human cells. 

The antigen must be surface expressed, preferably at high levels and
obviously not X-reactive to human.  MHC class I is a possibility, but
I am unsure of the haplotype since these cells were originally
derived from Swiss (outbred) mice.  Also constitutive expression of
MHC on fibroblasts can be quite low.

Any help here would be appreciated.
################################################################

 From: Dr Richard Darley, Ph.D.
LRF Differentiation Unit
Department of Haematology (A7)
University of Wales College of Medicine
Heath Park
Cardiff, CF4 4XN
U.K.
tel  (+44)  12 22 74 34 85
or (+44)  12 22 74 45 24
Fax (+44)  12 22 74 45 23
e-mail: darley@cf.ac.uk



This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:50:15 EST