I'm looking for an antibody which will distinguish NIH 3T3 cells from human cells. The antigen must be surface expressed, preferably at high levels and obviously not X-reactive to human. MHC class I is a possibility, but I am unsure of the haplotype since these cells were originally derived from Swiss (outbred) mice. Also constitutive expression of MHC on fibroblasts can be quite low. Any help here would be appreciated. ################################################################ From: Dr Richard Darley, Ph.D. LRF Differentiation Unit Department of Haematology (A7) University of Wales College of Medicine Heath Park Cardiff, CF4 4XN U.K. tel (+44) 12 22 74 34 85 or (+44) 12 22 74 45 24 Fax (+44) 12 22 74 45 23 e-mail: darley@cf.ac.uk
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