BD has developed a procedure for no wash, no lyse staining of whole blood. It depends on multiple color staining in which one of the antibodies stains all of the leukocytes. This is usually CD45. You set up the flow cytometer to trigger on the fluorescence of CD45 for leukocytes, thus eliminating all of the red cells, since they do not fluoresce and, therefore, do not trigger the instrument. The leukocytes can then be displayed and gated as usual. I have only tried this a few times and it is difficult. The amount of blood used, the flow rate and the trigger threshold seem to be critical to prevent overwhelming the detectors and having a high coincidence of red cells and leukocytes, which alters the scatter pattern. I would suggest talking to someone at BD. Hope this helps. Good flowing, Tony Bakke
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