> I am trying to gather information from those who are using the Baxter or > Cellpro columns for isolation of Stem Cells. > > We are going to start some projects involving either of these coupled > with Flow Sorting to isolate stem cells. > > Any information you can provide as to the recovery/purity etc would be > helpful. I would rather not spend valuable time and money in months of > testing these products. > > Thanks > > Jim Houston > Flow Cytometry Specialist > Cell and Gene Therapy > St. Jude Children's Res. Hosp. > Jim, Both systems work pretty well. We have more experience with Baxter system. Some things to keep in mind. Recovery is usually around 60-70% of starting pool. Purity can be pretty variable. Seen reports with CellPro system of 10-90%. Few reports with Baxter note 70-95%. Final purity may also be related to method of mobilization. We have noted in patients mobilized with chemo+G-CSF, median purity is 99% (92-99.8%). Those few mobilized only with G-CSF achieve purities of 75-88%. This phenomena has been reported in a 1996 paper that was in J. Hematotherapy. Can't recall lead author right now. Recovery is somewhat dependant on the starting purity of the CD34+ cells. We have noted that if CD34% purity is below 0.5%, final recovery gets into the 30-50% range. This experience is based on clinical scale devices. Research sized reagent kits may give different results. Drop me a note if you have any specific questions.
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